A near-infrared xanthene fluorescence probe for monitoring peroxynitrite in living cells and mouse inflammation model

作者：Wu, Yongquan^*; Shi, Aiping; Li, Yuanyan; Zeng, Hong; Chen, Xiaoyong; Wu, Jie; Fan, Xiaolin^*

来源：Analyst, 2018, 143(22): 5512-5519.

DOI：10.1039/c8an01107a

• PHOTOINDUCED ELECTRON-TRANSFER
• DRUG-INDUCED HEPATOTOXICITY
• ENDOGENOUS PEROXYNITRITE
• SENSITIVE DETECTION
• RATIONAL DESIGN
• NITRIC-OXIDE
• REDOX CYCLES
• IN-VITRO
• MITOCHONDRIA
• GLUTATHIONE

Peroxynitrite (ONOO-) plays important roles in the regulation of many physiological and pathological processes, and an increase in its levels is related to numerous diseases. Thus, accurate detection of ONOO- in physiological conditions is imperative for elucidating its functions. However, studies on high signal-to-noise-ratio (SNR) fluorescence imaging of ONOO-in vivo for its detection are currently lacking. Thus, a novel NIR xanthene fluorescence probe (NOF2) for the endogenous detection of ONOO- is designed and synthesized. The fluorescence of the NOF2 probe is pre-quenched by the hydroxyl protection group of diphenyl phosphinate. Additionally, the NOF2 probe exhibits good selectivity and sensitivity for ONOO- with a low detection limit of 0.40 mu M. Importantly, the NOF2 probe displays good performances for the detection of endogenous ONOO- not only in living cells but also in a mouse inflammation model. This demonstrates its great potential for applications involving the detection of ONOO- both in vitro and in vivo to explore the roles of ONOO- in different physiological systems.

• 出版日期2018-11-21
• 单位赣南师范大学