科研之友
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摘要
Parasitic worms (helminths) are known to actively modulate host immune responses and inflammation. The aim of this study was to investigate if adult body fluid (ABF) from the helminth Ascaris suum has immunomodulatory effects on different subtypes of human monocyte-derived macrophages (M phi) in vitro. M phi s were exposed to A. suum ABF at different stages of their differentiation and/or polarization. M phi were first differentiated from monocytes into either uncommitted (M-), classically activated (M(GM-CSF)) or alternatively activated (M(MCSF)) phenotypes and then stimulated with lipopolysaccharide (LPS). ABF strongly suppressed LPS-induced TNF alpha, IL-6 and IL-10 secretion in M(GM-CSF)s, however in M(M-CSF)s only INF-alpha was suppressed, with these cells secreting high levels of IL-10 which was not affected by ABF treatment. To determine if ABF modulated the differentiation of previously uncommitted M phi to either type 1 or type 2 M phi monocytes were differentiated with human serum into (M-)s and then polarized by IFN-gamma/LPS or IL-4 treatment in the presence of ABF. Under these conditions, ABF did not modulate cytokine secretion but did reduce CD80 expression in IFN gamma/LPS-polarized cells but not IL-4-polarized cells. Finally, we demonstrate that when monocytes are differentiated into M(GMCSF)s in the presence of ABF, subsequent inflammatory responses are markedly suppressed. Our data suggest that ABF inhibits cytokine secretion and co-stimulatory molecule expression in classically activated M phi but not in alternatively activated MC indicating selective action of ABF depending on M phi subtype. Moreover, ABF appears to exert stronger activity when acting upon M phi that have already been polarized to the type 1 phenotype, rather than influencing the polarization process per se.
- 出版日期2018-5
