Analysis of Pterins in Urine by HILIC

作者：Shi Zhi Guo; Wu Yu Bo; Luo Yan Bo; Feng Yu Qi^*

来源：Chromatographia, 2010, 71(9-10): 761-768.

DOI：10.1365/s10337-010-1567-0

• Column liquid chromatography
• Hydrophilic interaction chromatography
• Urine
• Neopterin
• Biopterin
• Isoxanthopterin

Pterins are a class of compounds excreted in urine. Levels of the pterins are found to be significantly elevated in a variety of diseases. A new method involving hydrophilic interaction chromatography with fluorescence detection has been developed for analysis of neopterin, biopterin, and isoxanthopterin in urine samples. Separation of these pterins on an aminopropyl hydrophilic interaction column was achieved by isocratic elution. The effects of the organic modifier content, ionic strength, and pH of the mobile phase on the hydrophilic behavior of the pterins were studied and the mechanism of their separation was also investigated. Under the optimum chromatographic conditions the linearity (r a parts per thousand yen 0.9995) and repeatability (relative standard deviation < 4.0%) of the method are good. Compared with reversed-phase high-performance liquid chromatography, the method is simple and convenient. The method was applied to the analysis of pterins in urine samples with satisfactory results.

• 出版日期2010-5
• 单位武汉大学