DEVELOPMENT OF MICROSATELLITE LOCI FOR CYCLOCARYA PALIURUS (JUGLANDACEAE), A MONOTYPIC SPECIES IN SUBTROPICAL CHINA

作者：Fan, Deng-Mei; Ye, Lin-Jiang; Luo, Yi; Hu, Wan; Tian, Shuang; Zhang, Zhi-Yong^*

来源：Applications in Plant Sciences, 2013, 1(6): 1200524.

DOI：10.3732/apps.1200524

• Cyclocarya paliurus
• Juglandaceae
• microsatellite marker
• population genetics

Premise of the study: Microsatellite loci were developed for a monotypic species endemic to subtropical China, Cyclocarya paliurus, to help infer the evolutionary histories of ancient monotypic genera in subtropical China.
Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, 28 primer sets were identified in two wild populations. All loci were polymorphic, with the number of alleles per locus ranging from two to eight. The expected and observed heterozygosities ranged from 0.153 to 0.802 and from 0 to 0.750, respectively. The transferability of the 28 primer pairs was tested on Juglans regia, Pterocarya stenoptera, and Platycarya strobilacea. Twenty-one (75.0%), 22 (78.6%), and 15 (53.6%) markers were successfully amplified in J. regia, P. stenoptera, and P. strobilacea, respectively.
Conclusions: These loci will be useful for in-depth analysis of genetic diversity and phylogeographical variation throughout the distribution range of C. paliurus.

• 出版日期2013-6
• 单位江西农业大学