NT-3-secreting human umbilical cord mesenchymal stromal cell transplantation for the treatment of acute spinal cord injury in rats

作者：Shang, Ai-Jia; Hong, Sun-Quan; Xu, Qiang^*; Wang, Hang-Yan; Yang, Ying; Wang, Zhong-Feng; Xu, Bai-Nan; Jiang, Xiao-Dan; Xu, Ru-Xiang

来源：Brain Research, 2011, 1391: 102-113.

DOI：10.1016/j.brainres.2011.03.019

• Spinal cord injury
• Transplantation
• Umbilical cord
• Mesenchymal stromal cells
• Nerve growth factors

An animal model for clip spinal cord injury (SCI) was used to determine whether Neurotrophin-3 (NT-3) genetically modified human umbilical mesenchymal stem cells (NT-3-HUMSCs) could promote the morphologic and functional recovery of injured spinal cords. Using the Basso, Beattie, and Bresnahan scores and a grid test, the rats in the HUMSC-treated and NT-3-HUMSCs groups had significantly improved locomotor functional recovery more than the control group. In comparison, the NT-3-HUMSCs group achieved better functional recovery than the HUMSCs group at the end of 12 weeks after SCI. The functional recovery was accompanied by increased intensity of 5-HT fibers, increased volume of spared myelination, and decreased area of the cystic cavity in the NT-3-HUMSCs group compared with the HUMSCs group. Moreover, transplanted NT-3-HUMSCs survived and produced larger amounts of NT-3 than the HUMSCs in the host spinal cord. These results show that NT-3 enhanced the therapeutic effects of HUMSCs after clip injury of the spinal cord.

• 出版日期2011-5-19
• 单位复旦大学; 中国人民解放军陆军总医院; 南方医科大学