Altered Na/Ca exchange distribution in ventricular myocytes from failing hearts

作者：Gadeberg Hanne C; Bryant Simon M; James Andrew F; Orchard Clive H^*

来源：American Journal of Physiology - Heart and Circulatory Physiology, 2016, 310(2): H262-H268.

DOI：10.1152/ajpheart.00597.2015

• cardiac myocytes
• T tubules
• intracellular Ca
• heart failure

In mammalian cardiac ventricular myocytes, Ca efflux via Na/Ca exchange (NCX) occurs predominantly at T tubules. Heart failure is associated with disrupted t-tubular structure, but its effect on t-tubular function is less clear. We therefore investigated t-tubular NCX activity in ventricular myocytes isolated from rat hearts similar to 18 wk after coronary artery ligation (CAL) or corresponding sham operation (Sham). NCX current (I-NCX) and L-type Ca current (I-Ca) were recorded using the whole cell, voltage-clamp technique in intact and detubulated (DT) myocytes; intracellular free Ca concentration ([Ca](i)) was monitored simultaneously using fluo-4. I-NCX was activated and measured during application of caffeine to release Ca from sarcoplasmic reticulum (SR). Whole cell I-NCX was not significantly different in Sham and CAL myocytes and occurred predominantly in the T tubules in Sham myocytes. CAL was associated with redistribution of I-NCX and I-Ca away from the T tubules to the cell surface and an increase in t-tubular I-NCX/I-Ca density from 0.12 in Sham to 0.30 in CAL myocytes. The decrease in t-tubular I-NCX in CAL myocytes was accompanied by an increase in the fraction of Ca sequestered by SR. However, SR Ca content was not significantly different in Sham, Sham DT, and CAL myocytes but was significantly increased by DT of CAL myocytes. In Sham myocytes, there was hysteresis between I-NCX and [Ca](i), which was absent in DT Sham but present in CAL and DT CAL myocytes. These data suggest altered distribution of NCX in CAL myocytes.

• 出版日期2016-1-15