A chemiluminescence enzyme immunoassay (CLEIA) for the determination of 19-nortestosterone residues in aquatic products

作者：Xu CL^*; Yu DH; Chu XG; Peng CF; Jin ZY

来源：Analytical Letters, 2006, 39(4): 709-727.

DOI：10.1080/00032710600611095

• growth promoters
• 19-nortestosterone
• enzyme immunoassay
• chemiluminescence

We developed a competitive chemiluminescent ( CL) enzyme immunoassay ( CLEIA) for rapid, sensitive analysis of 19-nortestosterone ( 19 beta-NT) in aquaculture tissues. Anti-19-NT polyclonal antibodies raised against protein conjugates of two haptenic derivatives of 19-NT were utilized in immobilized, antibody-based, competitive immunoassays. With homologous and heterologous ELISA systems, most antiserum titers fell in the range of 1: 3000-1: 100,000. Assay conditions, including concentrations of antisera and enzyme tracer, were optimized. The effect of pH and various blocking agents was also investigated. The optimized CLEIA system allowed 19-NT determination in the range of 0.07-30.5 ng/mL with apparent IC50 values of 1.2-2.8 ng/mL. Cross-reactivies with a number of compounds were determined, and the assay proved highly selective for 19-NT. The good recoveries achieved with different aquaculture tissue samples indicate that CLEIA is suitable for high-throughput screening of 19-NT in this kind of sample.

• 出版日期2006-3