New technique for mouse oocyte injection via a modified holding pipette

作者：Lyu, Q. F.; Deng, L.; Xue, S. G.; Cao, S. F.; Liu, X. Y.; Jin, W.; Wu, L. Q.^*; Kuang, Y. P.

来源：Reproductive BioMedicine Online, 2010, 21(5): 663-666.

DOI：10.1016/j.rbmo.2010.07.004

• holding pipette
• interspecies fertilization
• intracytoplasmic sperm injection
• mouse oocyte
• survival rate

To improve mouse oocyte survival from intracytoplasmic sperm injection, the sharp tip of the injection pipette has been modified to have a flat end. Here, for the same goal but for a more convenient manipulation, a sharp injection pipette was kept whereas the holding pipette was modified to have a trumpet-shaped opening, which allows deeper injection into the oocyte as it is held. Mouse oocyte injection with mouse and human spermatozoa was performed at 37 C. For the injection of mouse oocyte with mouse sperm head, a significantly higher survival rate (83%) was achieved by utilizing the modified holding pipette than the conventional one (21%; P < 0.001) and the fertilization rates were normal and comparable for both methods (82% versus 81%). A superior survival rate (82%) and acceptable normal fertilization rate (71%) were also achieved by utilizing the modified holding pipette for interspecies ICSI (injecting mouse oocyte with human spermatozoon). Taken together, by utilizing a holding pipette with a trumpet-shaped opening, acceptable rates of mouse oocyte survival and fertilization can be achieved using a sharp injection pipette under conditions usual for human oocyte injection.

• 出版日期2010-11
• 单位中南大学; 上海交通大学