Anticoagulant concentration, time and storage temperature on hematological parameters in automated blood count

作者：de Oliveira Aecio Carlos^*; Ribeiro Filho Jose Dantas; Guimaraes Jose Domingos; Ricardo e Silva Andre; Ferreira Dantas Waleska de Melo; Bonfa Laila de Paula; de Farias Sheila Kreutzfeld

来源：Ciencia Rural, 2010, 40(12): 2521-2526.

• dog
• hemogram
• EDTA
• time
• temperature

The study aimed to identify the effects of time, temperature of storage and excess of anticoagulant on hematological parameters of dogs. Blood samples of ten, clinically healthy dogs, of different breeds were utilized. Aliquots were stored with 1.8mg, 3.6mg, 7.2mg and 14.4mg of ethylenediaminetetraacetic acid (EDTA) per mL of blood, divided into two groups: 2 degrees C to 8 degrees C and room temperature. Right after collection, they were evaluated in four times: 0h, 12h, 24h and 48h. White blood cells, red blood cells, hemoglobin, packed cell volume (PCV), mean corpuscular volume (MCV), red cell distribution width (RDW), platelet and thrombocrit (PCT) were evaluated in the automatic cell counter. In the automatic cell counter analysis, the MCV increased significantly with higher concentrations of EDTA (7.2mg mL(-1) and 14.4mg mL(-1)) peaking at 2.36%, in the highest concentration. Temperature and time also influenced the MCV, which showed a decrease in time 12 under temperature of 2 degrees C to 8 C and an increase in time 24 and 48 at room temperature. Storage temperature influenced leukocytes and erythrocytes counts, which showed lower values at room temperature. Hemoglobin, PCV, platelet and PCT did not change significantly. The changes observed did not compromise the results obtained by automatic cell counter, showing that blood samples stored for two days remained in good condition for processing, especially when stored under refrigeration.

• 出版日期2010