Stability of RNA virus attenuation approaches

作者：Kenney Joan L; Volk Sara M; Pandya Jyotsna; Wang Eryu; Liang Xiaodong; Weaver Scott C^*

来源：Vaccine, 2011, 29(12): 2230-2234.

DOI：10.1016/j.vaccine.2011.01.055

• Vaccine stability
• RNA viruses
• Alphavirus
• Venezuelan equine encephalitis virus

The greatest risk from live-attenuated vaccines is reversion to virulence. Particular concerns arise for RNA viruses, which exhibit high mutation frequencies. We examined the stability of 3 attenuation strategies for the alphavirus, Venezuelan equine encephalitis virus (VEEV): a traditional, point mutation-dependent attenuation approach exemplified by TC-83; a rationally designed, targeted-mutation approach represented by V3526; and a chimeric vaccine, SIN/TC/ZPC. Our findings suggest that the chimeric strain combines the initial attenuation of TC-83 with the greater phenotypic stability of V3526, highlighting the importance of the both initial attenuation and stability for live-attenuated vaccines.

• 出版日期2011-3-9