A naturally occurring insertion in the RsFLC2 gene associated with late-bolting trait in radish (Raphanus sativus L.)

作者：Wang, Qingbiao; Zhang, Yongjie; Zhang, Li^*

来源：Molecular Breeding, 2018, 38(11): 137.

DOI：10.1007/s11032-018-0897-8

• Radish
• FLC2
• Bolting and flowering
• Intron variation
• QTL analysis

Premature flowering reduces the yield and quality of the harvested fleshy taproot in radish. However, there has been little molecular marker research on the radish late-bolting trait. In this study, F-2 and F-2:3 populations derived from a cross of Ninengo (late-bolting) and Maer (early-bolting) were analyzed to map late-bolting genes. Five hundred insertion and deletion (InDel) markers were designed according to the whole-genome resequencing data of the two parents. A genetic map was constructed based on the F-2 population, and a late-bolting gene was detected in a 1.1-cM region between the markers InDel520 and InDel535 on chromosome R02 that explained the highest (76.4%) phenotypic variance. RsFLC2 was identified as a candidate gene in this region. Notably, Ninengo contains a 1627-bp insertion near the 5 end of the first intron of RsFLC2. Allelic variation analyses in the F-2 population further validated that RsFLC2was associated with the late-bolting trait in radish. The expression pattern of RsFLC2 was significantly different between Ninengo and Maer during vernalization. Vernalization suppressed RsFLC2 expression, and the 1627-bp insertion in the first intron weakened gene repression in Ninengo plants, resulting in late-bolting. This study lays a foundation for uncovering the molecular mechanism of late-bolting and marker-assisted selection for breeding late-bolting varieties of radish.

• 出版日期2018-11
• 单位北京市农林科学院