Melatonin and a single-high dose methylprednisolone effect on the oxidant-antioxidant system in the rabbit heart tissue

作者：Mertoglu Cuma^*; Kiraz Zeynep Kusku; Sogut Erkan; Ozyurt Huseyin

来源：Turkish Journal of Biochemistry, 2015, 40(4): 316-322.

DOI：10.1515/tjb-2015-0019

• Methylprednisolone
• melatonin
• oxidative stres
• heart

Objective: Glucocorticoids have been used in the treatment of a number of diseases due to their widely therapeutic effects. But they have serious side effects. Although glucocorticoid therapy is an effective measure to prevent and treat many diseases in patients, it can cause adverse effects on the cardiovascular system secondary to oxidative stress. It has been suggested that melatonin is a potent antioxidant and that melatonin supplements may protect against such age-related diseases as atherosclerosis, cancer, and Alzheimer disease. In this study, we investigated whether such effects of a single high dose steroid has adverse consequences for the oxidant-antioxidant system of rabbit heart tissue and whether melatonin treatment is protective in the rabbit. For this purpose we measurument some biochemical parameters in the rabbit heart tissue.
Methods: Level of malondialdehyde (MDA, a lipid peroxidation product), protein carbonyl (PC, a protein oxidation product) and nitric oxide (NO) were measured from heart tissue samples of all rabbits included in the study. Also antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase activities were measured from heart tissues. In the study, 20 rabbits were used. The rabbits were separated into three groups. Group I: Control group, group II: Methylprednisolone group, group III: Methylprednisolone plus melatonin group.
Results: The results of the study; PC levels significantly decreased in methylprednisolone plus melatoninin group compared to the control group (p=0.03). PC levels decreased in methlyprednisolone group compared to the control group and higher than methlyprednisolone + melatonin group but these changes aren't significant statistically. No statistical difference was determined by MDA, NO levels and SOD, GSH-Px, catalase activities of all groups.
Conclusion: According to the results of this study we can say that administration of a single high dose methylprednisolone doesn't increase oxidative stress Also, melatonine is a potent antioxidant agent in the heart tissue.

• 出版日期2015