Monitoring the Differentiation and Migration Patterns of Neural Cells Derived from Human Embryonic Stem Cells Using a Microfluidic Culture System

作者：Lee Nayeon; Park Jae Woo; Kim Hyung Joon; Yeon Ju Hun; Kwon Jihye; Ko Jung Jae; Oh Seung Hun; Kim Hyun Sook; Kim Aeri; Han Baek Soo; Lee Sang Chul; Jeon Noo Li; Song Jihwan^*

来源：Molecules and Cells, 2014, 37(6): 497-502.

DOI：10.14348/molcells.2014.0137

• axons
• human embryonic stem cells
• microfluidics
• migration
• neural differentiation

Microfluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin-positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.

• 出版日期2014-6-30