A gene-specific non-enhancer sequence is critical for expression from the promoter of the small heat shock protein gene alpha B-crystallin

作者：Jing Zhe; Gangalum Rajendra K; Mock Dennis C; Bhat Suraj P^*

来源：Human Genomics, 2014, 8(1): 5.

DOI：10.1186/1479-7364-8-5

• Gene-specific promoter sequence
• Gene expression
• alpha B-crystallin
• Human retinal pigment epithelial cells
• Transgenic mice

Background: Deciphering of the information content of eukaryotic promoters has remained confined to universal landmarks and conserved sequence elements such as enhancers and transcription factor binding motifs, which are considered sufficient for gene activation and regulation. Gene-specific sequences, interspersed between the canonical transacting factor binding sites or adjoining them within a promoter, are generally taken to be devoid of any regulatory information and have therefore been largely ignored. An unanswered question therefore is, do gene-specific sequences within a eukaryotic promoter have a role in gene activation? Here, we present an exhaustive experimental analysis of a gene-specific sequence adjoining the heat shock element (HSE) in the proximal promoter of the small heat shock protein gene, alpha B-crystallin (cryab). These sequences are highly conserved between the rodents and the humans.
Results: Using human retinal pigment epithelial cells in culture as the host, we have identified a 10-bp gene-specific promoter sequence (GPS), which, unlike an enhancer, controls expression from the promoter of this gene, only when in appropriate position and orientation. Notably, the data suggests that GPS in comparison with the HSE works in a context-independent fashion. Additionally, when moved upstream, about a nucleosome length of DNA (-154 bp) from the transcription start site (TSS), the activity of the promoter is markedly inhibited, suggesting its involvement in local promoter access. Importantly, we demonstrate that deletion of the GPS results in complete loss of cryab promoter activity in transgenic mice.
Conclusions: These data suggest that gene-specific sequences such as the GPS, identified here, may have critical roles in regulating gene-specific activity from eukaryotic promoters.

• 出版日期2014-3-3