An indirect immunocolorimetric assay to detect rubella virus infected cells

作者：Chen Min hsin; Zhu Zhen; Zhang Yan; Favors Sheena; Xu Wen bo; Featherstone David A; Icenogle Joseph P^*

来源：Journal of Virological Methods, 2007, 146(1-2): 414-418.

DOI：10.1016/j.jviromet.2007.08.021

• detection of rubella virus
• Indirect immunocolorimetric assay
• noncytopathic

An indirect immunocolorimetric assay (ICA) to detect rubella virus infected cells by the naked eye was developed. This assay was as sensitive and specific as an indirect immunofluorescent assay (IFA), could detect as little as 10 plaque forming units (pfu) of rubella virus in the initial inoculum and could detect viruses from throat swabs. This assay detected infection with viruses of all nine rubella virus genotypes available for testing. It could be utilized for virus quantitation and a neutralization assay. In addition to detecting rubella virus infection, this assay also allowed us to confirm measles virus infection. This assay should be useful for virus isolation from clinical samples.

• 出版日期2007-12
• 单位中国疾病预防控制中心