Antioxidant responses of lentil and barley plants to boron toxicity under different nitrogen sources

作者：Tepe Muammer^*; Aydemir Tulin

来源：African Journal of Biotechnology, 2011, 10(53): 10882-10891.

• Boron toxicity
• antioxidant enzymes
• nitrate
• ammonium
• urea
• lentil
• barley

In this study, the effects of different nitrogen sources on lentil (Lens clunaris) and barley (Hordeum vulgare) plants, exposed to 5 and 10 mM boron stress previously, were studied. After ten-day germination, the lentil (native) and barley (Tokak157/37) were incubated 16 h light and 8 h dark per day for 7-day growth cycle under the conditions of boron stress via different nitrogen sources (10 mM nitrogen in NH(4) Cl, KNO(3) and urea). As a result of the changes in the nitrogen sources of the plants, there were determined decreases in the relative growth rate (%) and total chlorophyll content related to boron stress, (p < 0.05) and (p < 0.01), respectively. The changes in the lentil were obtained much more than those in the barley. In addition, the changes in the groups in which NH(4)(+) was used as nitrogen source were obtained at lowest levels. The concentrations of MDA, H(2)O(2) and proline showed increases under boron stress (p < 0.05). The effect of boron toxicity on the activities of SOD, GPX and LOX was similar in the two species but the levels of CAT and APX activities were different in both species under 5 and 10 mM boron stress (p < 0.01). SOD, GPX and LOX activities increased in the roots and shoots of boron treated plants as compared with the controls (p < 0.01). Although, lentil CAT and APX, activities decreased; in barley, CAT and APX, activities increased under boron toxicity. In conclusion, the fertilizers which contain NH(4)(+) should be used in the boron stressed farmlands.

• 出版日期2011-9-14