AbDesign: An algorithm for combinatorial backbone design guided by natural conformations and sequences

作者：Lapidoth Gideon D; Baran Dror; Pszolla Gabriele M; Norn Christoffer; Alon Assaf; Tyka Michael D; Fleishman Sarel J^*

来源：Proteins: Structure, Function, and Genetics , 2015, 83(8): 1385-1406.

DOI：10.1002/prot.24779

• CDRs
• V(D)J recombination
• computational protein design
• fuzzy-logic design
• Rosetta
• canonical conformations
• modular segments
• conformation-sequence optimization

Computational design of protein function has made substantial progress, generating new enzymes, binders, inhibitors, and nanomaterials not previously seen in nature. However, the ability to design new protein backbones for functionessential to exert control over all polypeptide degrees of freedomremains a critical challenge. Most previous attempts to design new backbones computed the mainchain from scratch. Here, instead, we describe a combinatorial backbone and sequence optimization algorithm called AbDesign, which leverages the large number of sequences and experimentally determined molecular structures of antibodies to construct new antibody models, dock them against target surfaces and optimize their sequence and backbone conformation for high stability and binding affinity. We used the algorithm to produce antibody designs that target the same molecular surfaces as nine natural, high-affinity antibodies; in five cases interface sequence identity is above 30%, and in four of those the backbone conformation at the core of the antibody binding surface is within 1 angstrom root-mean square deviation from the natural antibodies. Designs recapitulate polar interaction networks observed in natural complexes, and amino acid sidechain rigidity at the designed binding surface, which is likely important for affinity and specificity, is high compared to previous design studies. In designed anti-lysozyme antibodies, complementarity-determining regions (CDRs) at the periphery of the interface, such as L1 and H2, show greater backbone conformation diversity than the CDRs at the core of the interface, and increase the binding surface area compared to the natural antibody, potentially enhancing affinity and specificity. Proteins 2015; 83:1385-1406.

• 出版日期2015-8