Assessing the use of cutinase reversed micellar catalytic system for the production of biodiesel from triglycerides

作者：Badenes Sara M; Lemos Francisco; Cabral Joaquim M S^*

来源：Journal of Chemical Technology and Biotechnology, 2010, 85(7): 993-998.

DOI：10.1002/jctb.2393

• biodiesel
• cutinase
• reversed micelles
• transesterification
• methanolysis

BACKGROUND: This work evaluates the use of a reversed micellar catalytic system containing a lipolytic biocatalyst, cutinase, to promote the reaction of triglycerides with methanol, ethanol or butanol for the production of biodiesel in isooctane.
RESULTS: The specific activity of cutinase increased with increase of the alcohol chain length, and methanol showed a significant toxicity toward the enzyme leading to a specific activity decrease for high concentrations of this alcohol, which did not occur when ethanol or butanol were used. The composition of the mixture was analyzed and it was shown that, although the reaction could proceed without water, the presence of water allowed higher reaction rates and higher conversions, probably because water helps to maintain both the micellar structure and lipase conformation. Excess water, however, had a deleterious effect on conversion.
CONCLUSION: The influence of water and methanol, apart from micelle structure, was found to be linked to partition effects of diglyceride and monoglyceride, which may also have a significant impact on the reaction rate.

• 出版日期2010-7