Activation of spinal NF-B-kappa mediates pain behavior induced by plantar incision

作者：Yang Tao; Yang Pei^*; Jiang Liu Ming; Zhou Ri Yong

来源：International Journal of Clinical and Experimental Medicine, 2015, 8(6): 9149-9155.

• Nuclear factor kppa B (NF-B-kappa)
• plantar incision
• analgesia

A growing body of evidence indicates that the activation of nuclear factor kappa B (NF-B-kappa) pathway was involved in neuropathic and inflammatory pain, however, the role of NF-B-kappa in incisional pain is still unclear. Therefore, in this study, we investigated whether the activation of NF-B-kappa in the spinal cord is involved in pain hypersensitivity after a plantar incision in the rat hind paw. After rats received a plantar incision surgery, mechanical allodynia and thermal hyperalgesia were determined by von Frey filaments and radiant heat, respectively. Western blot was used to determineNF-B-kappa activation at different time points after incision. The NF-B-kappa inhibitor pyrrolidinedithiocarbamate (PDTC) was administered intrathecally 30 min before hind paw plantar incision to determine the role of NF-B-kappa in incision-induced pain. Our results showed that the expression level of NF-B-kappa was significantly increased in spinal cord dorsal horn from 30 min to 3 days after the incision. Intrathecal pretreatment of PDTC attenuated incision-induced mechanical allodynia and thermal hyperalgesia. Furthermore, PDTC significantly reduced the expression level of c-Fos in the dorsal horn after plantar incision. Taken together, plantar incision-induced pain behaviors can be prevented by the NF-B-kappa inhibitor. Our results suggest that the blockage of the NF-B-kappa signaling pathway might represent a valuable alternative for treating postoperative pain.

• 出版日期2015
• 单位温州医科大学