Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes

作者：Shin Seung Chul; Ahn Do Hwan; Kim Su Jin; Lee Hyoungseok; Oh Tae Jin; Lee Jong Eun; Park Hyun^*

来源：PLos One, 2013, 8(7): e68824.

DOI：10.1371/journal.pone.0068824

Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution.

• 出版日期2013-7-23
• 单位中国极地研究中心