Analysis of enzymetic hydrolysis of Ganoderma lucidum protein and antioxidation of its hydrolysates

作者：Huang, Sheng-Quan; Ao, Hong; Zeng, Fan-Kui; Yang, Bo

来源：Modern Food Science and Technology, 2013, 29(1): 24-28.

• Alcalase
• Anti-oxidant activities
• Anti-oxidation
• Free amino acids
• Ganoderma Lucidum
• Hydroxyl radicals
• Proteinase
• Reducing power

Commercial proteases Alcalase and Protamex were used to hydrolysis Ganoderma lucidum protein, the antioxidant activity of Ganoderma lucidum protein before and after hydrolysis were evaluated. The results showed that the free amino acid content increased from 0.15 mg/mL to 0.61 and 0.54 mg/mL when use Alcalase and Protamex as catalyzer. The main free amino acid were Lys, Val, Leu, Thr, Phe, Ala Arg, Ile, Cys, His and Try when treated with Protamex, while the main free amino acid were Ala, Val, Glu, Leu, Arg, Try and Thr when treated with Alcalase. Before hydrolysis, the hydroxyl radical scavenging rate of Ganoderma lucidum protein was 28.70%, increased to 39.10% and 33.30% when treated with Alcalase and Protamex respectively. The reducing power also increased significantly and the OD 700 was 0.21 before hydrolysis, being increased to 0.35 and 0.34 after hydrolysis.

• 出版日期2013-1
• 单位中国热带农业科学院椰子研究所; 华南理工大学