科研之友
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摘要
Electropenetrography (EPG) was used to study the feeding preference of Brevicoryne brassicae Linnaeus (Hemiptera: Aphididae) on Brassica napus L. We assessed the probing behaviors of B. brassicae on three B. napus cultivars (Qinyou10, Zhongheza88, and Qinyou79) at three developmental stages (two leaf, four leaf, and six leaf stages). The four leaf treatment had a shorter duration of pathway phase and a longer time to first intracellular stylet punctures on Qinyou10 compared to other cultivars, and the longest total ingestion duration on Qinyou10 and the shortest on Qinyou79, indicating a preference for Qinyou10 over Qinyou79. This cultivar preference is also reflected in the field with higher aphid densities on Qinyou10 in the previous investigation. In the two leaf treatment, we found a longer total probing duration and a shorter non-probing duration on Zhongheza488 compared to Qinyou10. In the six leaf treatment, we found a longer duration in time to the first probe, more probes, and longer duration of non-probing on Qinyou10 compared to other cultivars. However, phloem associated behaviors were not significantly influenced by cultivar in the latter two treatments. We conclude that future research should focus on phloem associated behaviors on the four leaf stage of B. napus in order to evaluate cultivars resistant to B. brassicae.
