An LC-MRM method for measuring intestinal triglyceride assembly using an oral stable isotope-labeled fat challenge

作者：Li Xiaofang; Parks Elizabeth J; McLaren David G; Lambert Jennifer E; Cardasis Helene L; Chappell Derek L; McAvoy Thomas; Salituro Gino; Alon Achilles; Dennie Justin; Chakravarthy Manu; Shankar Sudha S; Laterza Omar F; Lassman Michael E^*

来源：Bioanalysis, 2016, 8(12): 1265-1277.

DOI：10.4155/bio-2016-0024

• fractional appearance rate
• isotope-label
• MS
• precursor pool
• triglycerides

Aim: A traditional oral fatty acid challenge assesses absorption of triacylglycerol (TG) into the periphery through the intestines, but cannot distinguish the composition or source of fatty acid in the TG. Stable isotope-labeled tracers combined with LC-MRM can be used to identify and distinguish TG synthesized with dietary and stored fatty acids. Results: Concentrations of three abundant TGs (52: 2, 54: 3 and 54: 4) were monitored for incorporation of one or two H-2(11)-oleate molecules per TG. This method was subjected to routine assay validation and meets typical requirements for an assay to be used to support clinical studies. Conclusion: Calculations for the fractional appearance rate of TG in plasma are presented along with the intracellular enterocyte precursor pool for 12 study participants.

• 出版日期2016-6