Molecular structure and characterization of the cytokine TWEAK and its receptor Fn14 in bovine

作者：Zhang Jia Xin; Sang Ming; Li Jian Feng; Zhao Wei; Ma Hong Wei; Min Cui; Hu Yun Long; Du Ming Xiang; Zhang Shuang Quan^*

来源：Veterinary Immunology and Immunopathology, 2011, 144(3-4): 238-246.

DOI：10.1016/j.vetimm.2011.09.002

• TWEAK and Fn14
• Real-time Q-PCR
• Bioinformatics analyses
• 3D structure and binding sites
• The TNF ligand/receptor interaction

Full-length cDNA encoding TWEAK and Fn14 from bovine was isolated. We used bioinformatics to analyze the gene structure, function, evolutionary relationships, and predicted three-dimensional structure of proteins and binding sites. Real-time quantitative PCR (Q-PCR) analysis revealed that both TWEAK and Fn14 are constitutively expressed in various tissues in bovine. Bovine TWEAK and Fn14 interaction analysis by yeast two-hybrid. Our results suggest that the TWEAK-Fn14 pathway is evolutionarily highly conserved. It will be helpful for investigation on the biological role of the TWEAK/Fn14 system in this important animal model. Furthermore, it provides insight into the molecular evolution of the emerging TWEAK and Fn14 families.

• 出版日期2011-12-15
• 单位南京师范大学