A two-photon fluorescent probe for real-time monitoring of autophagy by ultrasensitive detection of the change in lysosomal polarity

作者:Jiang, Jiacheng; Tian, Xiaohe; Xu, Changzhi; Wang, Shuxin; Feng, Yan; Chen, Man; Yu, Haizhu; Zhua, Manzhou; Meng, Xiangming*
来源:Chemical communications, 2017, 53(26): 3645-3648.
DOI:10.1039/c7cc00752c

摘要

The first two-photon probe, Lyso-OC, was proposed for use in monitoring cell autophagy by detection of the change in the lysosomal polarity during the membrane fusion process of autophagy. The Lyso-OC probe exhibited desirable optical properties and a detection signal that was selective to the polarity change. More importantly, Lyso-OC displayed real-time monitoring of autophagy in living cells.