Multicolored Protein Nanoparticles: Synthesis, Characterization, and Cell Uptake

作者：Stromer Bobbi S; Roy Sonali; Limbacher Melissa R; Narzary Bardwi; Bordoloi Manobjyoti; Waldman Julia; Kumar Challa Vijaya^*

来源：Bioconjugate Chemistry, 2018, 29(8): 2576-2585.

DOI：10.1021/acs.bioconjchem.8b00282

Synthesis, characterization, and applications of strongly fluorescent, multicolored protein nanoparticles (GlowDots) are reported here. Bovine serum albumin was cross-linked under controlled conditions to form nano particles, where particle size was controlled from 20 to 100 +/- 10 nm by choosing appropriate reaction conditions. The absorption as well as the emission wavelengths were controlled without changing the particle size, unlike quantum dots. Each GlowDot was loaded with up to 214 +/- 50 chromophores, and hence, the particles have high molar absorptivities (10(6) M-1 cm(-1)) as well as high brightness (10(5) to 10(6) M-1 cm(-1)). A large number of functional groups cover the particle surface and these are further functionalized to enhance cellular uptake. GlowDots that were labeled with fluorescein and functionalized with taurine, for example, were quickly taken up by HeLa, MDA-MB-231, PC3, and L6 myoblast cells, as interrogated by fluorescence imaging studies. GlowDots were biocompatible, size tunable, biodegradable, strongly fluorescent, and stable for months at room temperature, and they may serve as substitutes for quantum dots in a variety of practical applications.

• 出版日期2018-8