Definition of a consensus integrin adhesome and its dynamics during adhesion complex assembly and disassembly

作者：Horton Edward R; Byron Adam; Askari Janet A; Ng Daniel H J; Millon Fremillon Angelique; Robertson Joseph; Koper Ewa J; Paul Nikki R; Warwood Stacey; Knight David; Humphries Jonathan D; Humphries Martin J

来源：Nature Cell Biology, 2015, 17(12): 1577-1587.

DOI：10.1038/ncb3257

Integrin receptor activation initiates the formation of integrin adhesion complexes (IACs) at the cell membrane that transduce adhesion-dependent signals to control a multitude of cellular functions. Proteomic analyses of isolated IACs have revealed an unanticipated molecular complexity; however, a global view of the consensus composition and dynamics of IACs is lacking. Here, we have integrated several IAC proteomes and generated a 2,412-protein integrin adhesome. Analysis of this data set reveals the functional diversity of proteins in IACs and establishes a consensus adhesome of 60 proteins. The consensus adhesome is likely to represent a core cell adhesion machinery, centred around four axes comprising ILK PINCH kindlin, FAK paxillin, talin vinculin and alpha-actinin zyxin VASP, and includes underappreciated IAC components such as Rsu-1 and caldesmon. Proteomic quantification of IAC assembly and disassembly detailed the compositional dynamics of the core cell adhesion machinery. The definition of this consensus view of integrin adhesome components provides a resource for the research community.

• 出版日期2015-12