A new kind of aptamer-based immunomagnetic electrochemiluminescence assay for quantitative detection of protein

作者：Zhu, Debin; Zhou, Xiaoming; Xing, Da^*

来源：Biosensors and Bioelectronics, 2010, 26(1): 285-288.

DOI：10.1016/j.bios.2010.06.028

• Aptamer
• Immunomagnetic
• electrochemiluminescence
• Protein
• Platelet-derived growth factor B-chain

A new kind of aptamer-based immunomagnetic electrochemiluminescence (1M-ECL) assay for quantitative detection of protein is developed The assay consists of a double aptamer sandwich format in which a biotin-labeled aptamer is used for rapid and specific capture of target protein, and a [Ru(bpy)(3)](2+) (TBR)-labeled aptamer is used for ECL detection As an example, platelet-derived growth factor B-chain homodimer (PDGF-BB) was detected by the method Experimental results show that the detection limit of the assay is 80 pmol/L of PDGF-BB A calibration curve with a linearity range from 0 1 to 1000 nmol/L is established, thus, make quantitative analysis possible The method has been used to detect PDGF-BB in fetal calf serum

• 出版日期2010-9-15
• 单位华南师范大学