Acetylation of MyoD directed by PCAF is necessary for the execution of the muscle program

作者：Sartorelli V^*; Puri PL; Hamamori Y; Ogryzko V; Chung G; Nakatani Y; Wang JYJ; Kedes L

来源：Molecular Cell, 1999, 4(5): 725-734.

DOI：10.1016/S1097-2765(00)80383-4

p300/CBP and PCAF coactivators have acetyltransferase activites and regulate transcription, cell cycle progression, and differentiation. They are both required for MyoD activity and muscle differentiation. Nevertheless, their roles must be different since the acetyltransferase activity of PCAF but not of p300 is involved in controlling myogenic transcription and differentiation. Here, we provide a molecular explanation of this phenomenon and report that MyoD is directly acetylated by PCAF at evolutionarily conserved lysines. Acetylated MyoD displays an increased affinity for its DNA target. Importantly, conservative substitutions of acetylated lysines with nonacetylatable arginines impair the ability of MyoD to stimulate transcription and to induce muscle conversion indicating that acetylation of MyoD is functionally critical.

• 出版日期1999-11
• 单位河北医科大学