MR Imaging of Inflammation during Myelin-Specific T Cell-Mediated Autoimmune Attack in the EAE Mouse Spinal Cord

作者：Robinson Kristine M; Njus Jeffrey M; Phillips Daniel A; Proctor Thomas M; Rooney William D; Jones Richard E^*

来源：Molecular Imaging and Biology, 2010, 12(3): 240-249.

DOI：10.1007/s11307-009-0272-6

• MRI
• EAE
• Feridex
• Ferumoxide
• Multiple sclerosis

The purpose of this study is to detect myelin-specific T cells, key pathological mediators in early multiple sclerosis, and the corresponding animal model, experimental autoimmune encephalomyelitis (EAE), in the mouse spinal cord.
T cells were labeled with the iron-based, magnetic resonance (MR) contrast reagent, Feridex, and the transfection reagent, protamine sulfate, resulting in similar to 100% iron-labeling efficiency. Feridex-labeling did not alter the induction of EAE by T cells, and recipients were imaged by a 12-T MR instrument.
Focal hypointense lesions were resolvable to gray or white matter of the lumbar spinal cord in T(2)-weighted images of the recipients of Feridex-labeled T cells. Lesions corresponded to histological evidence of inflammatory lesions and iron-labeled cells in eight-of-eight mice. In contrast, hypointense lesions were not observed eight-of-eight recipients of unlabeled T cells.
These results demonstrate and provide methodologies for labeling, detecting, and extracting MRI-detectable foci of iron-labeled cells.

• 出版日期2010-6