A novel metallobridged bis(beta-cyclodextrin)s fluorescent probe for the determination of glutathione

作者：Tang Bo^*; Liu Fang; Xu Kehua; Tong Lili

来源：FEBS Journal, 2008, 275(7): 1510-1517.

DOI：10.1111/j.1742-4658.2008.06310.x

• competitive complexation
• glutathione
• metallobridged bis(beta-cyclodextrin)s
• molecular recognition
• spectrofluorimetry

A novel metallobridged bis(beta-cyclodextrin)s 2 [bis(beta-CD)s 2] was synthesized and characterized by means of H-1 NMR, IR, element analysis and redox iodometric titration. The fluorescence of metallobridged bis(beta-CD)s 2 was weak compared with bis(beta-CD)s 1 because of the paramagnetism of copper (II) ions. Glutathione was able to form complexes with copper (II) derived from the metallobridged bis(beta-CD)s 2. This competitive complexation with copper (II) may lead to a significant fluorescence recovery of the bis(beta-CD)s. Therefore, a rapid and simple spectrofluorimetric method was developed for the determination of glutathione. The analytical application for glutathione was investigated in NaCl/P-i (pH 6.00) at room temperature. The linear range of the method was 0.30-20.0 mu mol.L-1 with a detection limit of 63.8 nmol.L-1. There was no interference from the plasma constituents. The proposed method had been successfully used to determine glutathione in human plasma.

• 出版日期2008-4
• 单位山东师范大学