The mechanism of HTLV-1 LTR activation by TPA varies in different human T-cell lines: Role of specific PKC isoforms

作者：Chamias Rachel; Huleihel Mahmoud; Aboud Mordechai^*

来源：Leukemia Research, 2010, 34(1): 93-99.

DOI：10.1016/j.leukres.2009.03.032

• HTLV-1 LTR
• 21 bp TRE
• ERR-1
• Sp1 site
• TPA
• PKC isoforms
• Jurkat and H9 T-cell lines

We demonstrate here that TPA activates HTLV-1 LTR expression in Jurkat and H9 T-cell lines, by strictly different mechanisms. In Jurkat cells this activation is exerted by a PKC alpha- and PKC epsilon-antagonized mechanism which operates through an Sp1 binding site residing within the Est responsive region 1 of the LTR. On the other hand, in H9 cells TPA activates the LTR by two consecutive mechanisms; the first depends on PKC eta activity and is exerted through the 21 bp repeats of the LTR, whereas the second is analogous to that observed in Jurkat cells, except that it is antagonized by PKC delta.

• 出版日期2010-1