AMPEROMETRIC GLUCOSE BIOSENSOR BASED ON ELECTROPOLYMERIZED CARBON NANOTUBE/POLYPYRROLE COMPOSITE FILM

作者：Branzoi Viorel^*; Pilan Luisa; Branzoi Florina

来源：Romanian Journal of Chemistry, 2009, 54(10): 783-+.

• nanocomposite films
• polypyrrole
• carbon nanotubes
• amperometric biosensor
• glucose

In this paper we report a one-step preparation procedure of an amperometric biosensor for glucose based on incorporating single-walled carbon nanotubes (SWCNTs) that were covalently functionalized with a water soluble conducting polymer, poly (m-aminobenzene sulfonic acid (SWCNT-PABS) and the glucose oxidase (GOD) within an electropolymerized polypyrrole (PPY) film. The nanocomposite films were electrochemically synthesized by clectrooxidation of pyrrole in an aqueous solution containing appropriate amounts of GOD and CNTs. The amperometric detection of glucose was assayed by potentiostating the enzyme electrode at a potential to oxidize the enzymatically produced H(2)O(2) with minimal interference from the coexisting electroactive compounds. The results indicate that the PPY-CNTs-GOD nanobiocomposite film is highly sensitive and suitable for glucose biosensor function. The biosensor exhibits excellent response performance to glucose with a linear range from 0.5 to 50 mM. Furthermore, the biosensor shows rapid response, high sensitivity and long-term stability.

• 出版日期2009-10