An integrative process of bioconversion of oil palm empty fruit bunch fiber to ethanol with on-site cellulase production

作者：Zhu Youshuang; Xin Fengxue^*; Zhao Ying; Chang Yunkang

来源：Bioprocess and Biosystems Engineering, 2014, 37(11): 2317-2324.

DOI：10.1007/s00449-014-1209-2

• Cellulase
• Organosolv pretreatment
• OPEFB
• Solid state fermentation
• Ethanol

The aim of this study was to efficiently convert oil palm empty fruit bunch fiber (OPEFB), one of the most commonly generated lingo-wastes in Southeast Asia, into both cellulase and bioethanol. The unprocessed cellulase crude (37.29 %) produced under solid-state fermentation using OPEFB as substrate showed a better reducing sugar yield using filter paper than the commercial enzyme blend (34.61 %). Organosolv pretreatment method could efficiently reduce hemicellulose (24.3-18.6 %) and lignin (35.2-22.1 %) content and increase cellulose content (40.5-59.3 %) from OPEFB. Enzymatic hydrolysis of pretreated OPEFB using the crude cellulase with 20 % solid content, enzyme loading of 15 FPU/g OPEFB at 50 A degrees C, and pH 5.5 resulted in a OPEFB hydrolysate containing 36.01 g/L glucose after 72 h. Fermentation of the hydrolysate medium produced 17.64 g/L ethanol with 0.49 g/g yield from glucose and 0.088 g/g yield from OPEFB at 8 h using Saccharomyces cerevisiae.

• 出版日期2014-11
• 单位济宁医学院