Development of an analytical protocol for the estimation of vitamin D-2 in fortified toned milk

作者：Kaushik Ravinder; Sachdeva Bhawana; Arora Sumit^*; Wadhwa Balbir Kaur

来源：Food Chemistry, 2014, 151: 225-230.

DOI：10.1016/j.foodchem.2013.11.085

• Fortified milk
• HPLC
• Vitamin D-2 estimation

A simple and rapid method has been developed for the isolation and determination of vitamin D-2 in fortified milk samples by HPLC. The advantage of the proposed method is that sample preparation time is reduced and both crystalline and encapsulated forms of vitamin D-2 can be estimated. The developed protocol involves a few extraction steps and requires less amounts of reagents. The HPLC separation of vitamin D-2 was carried out on a reverse phase C-18 column with photo diode array detector at 254 nm. Some additional steps were required for extraction of microencapsulated vitamin D-2 for breaking down coating material in comparison to crystalline vitamin D-2. The recovery of vitamin D-2 in fortified toned milk by the proposed method ranged from 96.46% to 99.05%. Non significant degradation was observed in vitamin D-2 during seven days storage and also under light exposure (1485 lux) for 32 h.

• 出版日期2014-5-15