Laminin-5 suppresses chondrogenic differentiation of murine teratocarcinoma cell line ATDC5

作者：Hashimoto J; Ogawa T; Tsubota Y; Miyazaki K^*

来源：Experimental Cell Research, 2005, 310(2): 256-269.

DOI：10.1016/j.yexcr.2005.07.025

• laminin-5
• gamma 2 chain
• chondrogenesis
• integrin
• ATDC5 cells

Laminin-5 is an important basement membrane protein that regulates cell adhesion and motility. It was previously found that the gamma 2 chain of laminin-5 is transiently expressed in embryonic cartilage. This suggests a possible role of laminin-5 in chondrogenesis. Here, we examined this possibility using the murine teratocarcinoma cell line ATDC5. ATDC5 cells transiently and weakly expressed laminin-5 when they were stimulated for differentiation. Exogenous laminin-5 in either insoluble or soluble form strongly inhibited the differentiation phenotypes, i.e. formation of cartilaginous cell aggregates and production of chondrogenic marker proteins through its integrin-binding domain LG3 in the U3 chain. Laminin-5 had no effect on cell growth. In addition, we found that the laminin-5 with the 105-kDa, processed gamma 2 chain suppressed differentiation more strongly than one with the 150-kDa gamma 2 chain. This indicated that the proteolytic processing of gamma 2 chain regulated the activity of laminin-5. However, a gamma 2 chain short arm fragment had no effect on the chondrogenesis, and it rather suppressed the differentiation at excessive concentrations. These results suggest that laminin-5 and its processing modulate chondrogenic differentiation during development.

• 出版日期2005-11-1