Molecular detection of viral agents in free-ranging and captive neotropical felids in Brazil

作者：Furtado Mariana M^*; Taniwaki Sueli A; de Barros Iracema N; Brandao Paulo E; Catao Dias Jose L; Cavalcanti Sandra; Cullen Laury; Filoni Claudia; de Almeida Jacomo Anah T; Jorge Rodrigo S P; Silva Nairleia dos Santos; Silveira Leandro; Ferreira Neto Jose S

来源：Journal of Veterinary Diagnostic Investigation, 2017, 29(5): 660-668.

DOI：10.1177/1040638717720245

• Carnivore protoparvovirus 1
• felid alphaherpesvirus 1
• feline immunodeficiency virus
• Herpairulus yagouaroundi
• Leopardus tigrinus
• Panthera onca
• Puma concolor

We describe molecular testing for felid alphaherpesvirus 1 (FHV-1), carnivore protoparvovirus 1 (CPPV-1), feline calicivirus (FCV), alphacoronavirus 1 (feline coronavirus [FCoV]), feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), and canine distemper virus (CDV) in whole blood samples of 109 free-ranging and 68 captive neotropical felids from Brazil. Samples from 2 jaguars (Panthera onca) and 1 oncilla (Leopardus tigrinus) were positive for FHV-1; 2 jaguars, 1 puma (Puma concolor), and 1 jaguarundi (Herpairulus yagouaroundi) tested positive for CPPV-1; and 1 puma was positive for FIV. Based on comparison of 103 nucleotides of the UL24-UL25 gene, the FHV-1 sequences were 99-100% similar to the FHV-1 strain of domestic cats. Nucleotide sequences of CPPV-1 were closely related to sequences detected in other wild carnivores, comparing 294 nucleotides of the VP1 gene. The FIV nucleotide sequence detected in the free-ranging puma, based on comparison of 444 nucleotides of the pol gene, grouped with other lentiviruses described in pumas, and had 82.4% identity with a free-ranging puma from Yellowstone Park and 79.5% with a captive puma from Brazil. Our data document the circulation of FHV-1, CPPV-1, and FIV in neotropical felids in Brazil.

• 出版日期2017-9