Multi-crystal native SAD analysis at 6 keV

作者：Liu Qun^*; Guo Youzhong; Chang Yanqi; Cai Zheng; Assur Zahra; Mancia Filippo; Greene Mark I; Hendrickson Wayne A

来源：Acta Crystallographica Section D-Biological Crystallography, 2014, 70(10): 2544-2557.

DOI：10.1107/S1399004714013376

Anomalous diffraction signals from typical native macromolecules are very weak, frustrating their use in de novo structure determination. Here, native SAD procedures are described to enhance signal to noise in anomalous diffraction by using multiple crystals in combination with synchrotron X-rays at 6 keV. Increased anomalous signals were obtained at 6 keV compared with 7 keV X-ray energy, which was used for previous native SAD analyses. A feasibility test of multicrystal-based native SAD phasing was performed at 3.2 angstrom resolution for a known tyrosine protein kinase domain, and real-life applications were made to two novel membrane proteins at about 3.0 angstrom resolution. The three applications collectively serve to validate the robust feasibility of native SAD phasing at lower energy.

• 出版日期2014-10