Membrane Crystals of Plant Light-Harvesting Complex II Disassemble Reversibly in Light

作者：Hind Geoffrey; Wall Joseph S; Varkonyi Zsuzsanna; Istokovics Anita; Lambrev Petar H; Garab Gyozo^*

来源：Plant and Cell Physiology, 2014, 55(7): 1296-1303.

DOI：10.1093/pcp/pcu064

• Granal thylakoid membranes
• LHCII
• Light-induced reorganizations
• Lipid-protein interactions
• Membrane crystal
• Thermo-optic effect

Using the mass-measuring capability of scanning transmission electron microscopy, we demonstrate that membrane crystals of the main light-harvesting complex of plants possess the ability to undergo light-induced dark-reversible disassociations, independently of the photochemical apparatus. This is the first direct visualization of light-driven reversible reorganizations in an isolated photosynthetic antenna. These reorganizations, identified earlier by circular dichroism (CD), can be accounted for by a biological thermo-optic transition: structural changes are induced by fast heat transients and thermal instabilities near the dissipation, and self-association of the complexes in the lipid matrix. A comparable process in native membranes is indicated by earlier findings of essentially identical kinetics, and intensity and temperature dependences of the Delta CD in granal thylakoids.

• 出版日期2014-7