Analysis of enteroendocrine cell populations in the human colon

作者：Martins Patricia; Fakhry Josiane; de Oliveira Enio Chaves; Hunne Billie; Fothergill Linda J; Ringuet Mitchell; Reis Debora d' Avila; Rehfeld Jens F; Callaghan Brid; Furness John B

来源：Cell and Tissue Research, 2017, 367(2): 161-168.

DOI：10.1007/s00441-016-2530-7

• Enteroendocrine cells
• Gastrointestinal hormones
• Human chemical coding

Recent studies have shown that patterns of colocalisation of hormones in enteroendocrine cells are more complex than previously appreciated and that the patterns differ substantially between species. In this study, the human sigmoid colon is investigated by immunohistochemistry for the presence of gastrointestinal hormones and their colocalisation. The segments of colon were distant from the pathology that led to colectomy and appeared structurally normal. Only four hormones, 5-hydroxytryptamine (5-HT), glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and somatostatin, were common in enteroendocrine cells of the human colon. Cholecystokinin, present in the colon of some species, was absent, as were glucose-dependent insulinotropic peptide, ghrelin and motilin. Neurotensin cells were extremely rare. The most numerous cells were 5-HT cells, some of which also contained PYY or somatostatin and very rarely GLP-1. Almost all GLP-1 cells contained PYY. It is concluded that enteroendocrine cells of the human colon, like those of other regions and species, exhibit overlapping patterns of hormone colocalisation and that the hormones and their patterns of expression differ between human and other species.

• 出版日期2017-2