Amperometric immunosensor for simultaneous detection of three analytes in one interface using dual functionalized graphene sheets integrated with redox-probes as tracer matrixes

作者：Zhu, Qiang; Chai, Yaqin^*; Yuan, Ruo; Zhuo, Ying; Han, Jing; Li, Ya; Liao, Ni

来源：Biosensors and Bioelectronics, 2013, 43: 440-445.

DOI：10.1016/j.bios.2012.12.030

• Amperometric immunosensor
• Simultaneous multianalytes immunoassays (SMIAs)
• Sandwich format immunoassay
• Dual functionalized graphene sheets
• Redox probe

A novel immunoassay protocol for simultaneous electrochemical determination of alpha-fetoprotein (AFP), carcinoembryonic (CEA) and streptococcus suis serotype 2 (SS2) was designed. As standard sandwich-type immunoassay format, three primary antibodies (Ab1), anti-CEA and anti-AFP and anti-SS2, were immobilized via protein A (PA) adsorbed by Nafion modified electrodes, and functionalized graphene sheets (GS), containing abundant gold nanoparticles (AuNPs) and carboxyl group, were used to immobilize secondary antibody@redox-probe so as to act as tracer. Concentration of each analyte was quantitatively related to the reduction peak current of corresponding redox-probe in differential pulse voltammetry (DPV) scan. The resulting immunosensor exhibited high selectivity and sensitivity in simultaneous determination of three analytes. The linear range was from 0.016 to 50 ng/mL for AFP with a detection limit of 5.4 pg/mL, 0.010 to 50 ng/mL for CEA with a detection limit of 2.8 pg/mL and 0.012 to 50 ng/mL for SS2 with a detection limit of 4.2 pg/mL (SIN=3).

• 出版日期2013-5-15
• 单位重庆师范大学; 西南大学