LIVE/DEAD CELL ASSAY BASED ON DIELECTROPHORESIS-ON-A-CHIP

作者：Iliescu Ciprian^*; Tresset Guillaume; Iliescu Florina S; Sterian Paul E

来源：Polytechnical University of Bucharest Scientific Bulletin Series A- Applied Mathematics and Physics, 2010, 72(1): 33-44.

• BioMEMS
• Dielectrophoresis
• Cell separation
• Microfluidics

The paper presents a field-flow separation method of particle populations in a dielectrophoretic (DEP) chip with asymmetric electrodes under continuous ,flow. The structure of the DEP device (with one thick electrode that defines the walls of the microfluidic channel and one thin electrode), as well as the fabrication and characterization of the device were previous described. A characteristic of this structure is Mat it generates an increased gradient of electric field it? the vertical plane that can levitate the particles experiencing negative DEP. The separation method consists of trapping one population to the bottom of the microfluidic channel using positive DEP, while the other population that exhibits negative DEP is levitated and flowed out. Viable and nonviable yeast cells were used for testing the separation method.

• 出版日期2010