Antifungal activity of extracts by supercritical carbon dioxide extraction from roots of Echinacea angustifolia and analysis of their constituents using gas chromatography-mass spectrometry (GC-MS)

作者：Li Dahui; Wang Zaigui; Zhang Yunhua^*

来源：Journal of Medicinal Plants Research, 2011, 5(23): 5605-5610.

• Antifungal activity
• Echinacea angustifolia DC
• gas chromatography-mass spectrometry (GC-MS)
• SC-CO(2) extraction

In this work the supercritical fluid extraction with carbon dioxide was applied to obtain the extracts from roots of Echinacea angustifolia DC. The extracts were evaluated through the antifungal in vitro assay against fungal strain Botrytis cinerea. Furthermore, the active compounds from the extracts were separated by macroporous resin and analyzed using gas chromatography-mass spectrometry (GC-MS). The results show that the extracts, obtained using SC-CO(2) extraction technique, had strong antifungal activity against B. cinerea with EC(50) and EC(90) values of 948 and 1869 mu g/ml, minimum inhibitory concentration (MIC) of 250 mu g/ml, and minimum fungicidal concentration (MFC) of 2000 mu g/ml, respectively. After separation with macroporous resin chromatography, nine fractions were collected from the SC-CO(2) extracts. At concentration of 2000 mu g/ml, fractions 3, 7, and 8 presented highly antifungal activity against B. cinerea, with inhibition ratio of 40.59, 73.04 and 53.37%, respectively. Determined by the GC-MS analysis, the composition of fraction 7 consisted of different kinds of volatile oils, most of which were ketones (82.71%) and unsaturated hydrocarbons (9.59%). The present study has provided the new evidence that the Echinacea extracts are involved in the antifungal activity.

• 出版日期2011-10-23
• 单位安徽农业大学