A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats

作者：Liu Wei; Dong Xue Song; Sun Yu Qiang; Liu Zhi^*

来源：International Journal of Clinical and Experimental Medicine, 2014, 7(4): 919-926.

• Septic shock
• acute kidney injury
• fluid resuscitation
• hydrogen inhalation
• oxidative stress
• inflammation

Objective: To investigate the therapeutic effects of a novel fluid resuscitation protocol (early fluid resuscitation plus 2% hydrogen inhalation) on acute kidney injury during septic shock induced by lipopolysaccharide in rats. Methods: Sixty male Wistar rats were randomly divided into four groups (n = 15 per group): control group (C), septic shock group (S), septic shock with early fluid resuscitation group (R), and septic shock with early fluid resuscitation plus 2% hydrogen inhalation group (R+H-2). The rats were ventilated, and a 2% hydrogen mixture was used in Group R+H-2. Lipopolysaccharide (10 mg/kg) was administered to establish the septic shock model in rats and fluid resuscitation was performed in Groups R and R+H-2. Results: Fluid resuscitation with 2% hydrogen inhalation decreased serum creatinine, blood urea nitrogen, and neutrophil gelatinase-associated lipocalin. It also reduced oxidative stress injury and decreased renal tumor necrosis factor-a and interleukin-6 levels compared with fluid resuscitation alone. Conclusion: Early fluid resuscitation plus 2% hydrogen inhalation provided more protection against AKI during septic shock.

• 出版日期2014
• 单位中国医科大学