科研之友
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摘要
As the fourth most important food crop, potato plays a key role in food safety and economic development of the world. Harvested potato tubers can be stored for a long time, but sprouting and cold-induced sweetening (CIS) can seriously affect the quality of tubers during storage. One of the key pathways involved in CIS is starch degradation, in which both alpha-amylase and beta-amylase play important roles. However, each amylase belongs to an extensive gene family and it is not clear which genes are the key regulators. In this study, we identified genes most likely regulating starch degradation. We first selected candidate genes from the public potato genome database and then investigated their expression patterns associated with reducing sugars and amylase activities. The results showed that the activity of alpha-amylase was mainly caused by StAmy23 and the activity of beta-amylase was mainly caused by StBAM1 and StBAM7. In addition, alpha-amylase and beta-amylase may play important roles in starch degradation of the tubers stored at low temperature and during sprouting, and the amylase activity may be regulated by the amylase inhibitor in cold-stored tubers.
