Development of a low-cost paper-based ELISA method for rapid Escherichia coli O157:H7 detection

作者：Pang, Bo; Zhao, Chao; Li, Li; Song, Xiuling^*; Xu, Kun; Wang, Juan; Liu, Yushen; Fu, Kaiyue; Bao, Hao; Song, Dandan; Meng, Xiangjun; Qu, Xiaofeng; Zhang, Zhuping; Li, Juan^*

来源：Analytical Biochemistry, 2018, 542: 58-62.

DOI：10.1016/j.ab.2017.11.010

• Paper-based ELISA
• Escherichia coli O157:H7
• On-site detection
• Foodbome pathogens

Escherichia coil O157:H7 (E. coil O157:H7) has become one of the most dangerous foodborne pathogenic bacteria around the world. Currently, because of the tedious, high-cost and stringent laboratory conditions required, the conventional E. coli O157: H7 detection methods, such as culture-based methods and polymerase chain reaction (PCR), have much limitation. Thus, we developed a novel paper-based enzyme-linked immunosorbent assay (p-ELISA) with shorter operation duration, lower cost, relatively higher sensitivity and wider application. This method required less than 3 h and 5 mu L of sample to complete the detection. The limit of detection (LOD) for E. coil O157:H7 reached 1 x 10(4) CFU/mL with high specificity. To be more suitable for on-site testing, the readout could be rapidly obtained without any expensive instruments. In this study, we chose E. coli O157:H7 as the representative, and our method could provide a platform for determination of other pathogenic bacteria.

• 出版日期2018-2-1
• 单位吉林大学