科研之友
微信
新浪微博
Facebook
分享链接
摘要
Rhododendron hybridum Hort. (Ericaceae) is an important ornamental species with striking continuous flowering features. However, few genomic resources are currently available for the species; thus, breeding programs are blocked by a lack of genetic information. Here, we document our transcriptomic profiling of four different R. hybridum tissues using whole transcriptome shotgun sequencing (RNA-Seq) to gain insight on functional genes, and to isolate expressed sequence tag-simple sequence repeat (EST-SSR) markers for breeding and conservation purposes. In total 38,050,296 high-quality sequence reads were obtained, and 56,120 unigenes (contiguous sequence assemblies representing transcripts, with N50 = 1,236 bp) were assembled. Of these, 32,580 (58.05%) and 8,788 (15.66%) were annotated to the GO and KEGG databases, respectively. Additionally, 38,775 (69.09%) and 37,409 (66.66%) of the R. hybridum unigenes aligned to the Arabidopsis thaliana and Oryza sativa genomes, respectively. A total of 21,103 SSR motifs were identified in 15,050 of the unigenes. Among them, dinucleotide repeats account for the largest proportion (49.27%), followed by mono- (35.94%) and trinucleotide repeats (21.5%). This is the first comprehensive transcriptome dataset for R. hybridum, and RNA-Seq technology is proven to be useful approach for EST-SSR development. Such a vast quantity of sequence data and microsatellite markers should prove to be robust tools for subsequent genomic research and breeding programs in R. hybridum and related species.
- 出版日期2018-4
- 单位平顶山学院
