A Novel beta-1,4-mannanase Isolated from Paenibacillus polymyxa KT551

作者：Hori Kazuyuki; Kawabata Yasuyuki; Nakazawa Yozo; Nishizawa Makoto; Toeda Kazuki^*

来源：Food Science and Technology Research, 2014, 20(6): 1261-1265.

DOI：10.3136/fstr.20.1261

• Paenibacillus polymyxa
• beta-1,4-mannanase
• mannanooligosaccharides
• beta-mannosidase
• alpha-galactosidase

A beta-1,4-mannanase producing bacterium was isolated from soil collected in Akita Prefecture, Japan. The bacterium was identified as Paenibacillus polymyxa KT551 and was shown to produce a novel beta-1,4-mannanase. The novelty of the enzyme was established by its N-terminal amino acid sequence, molecular weight and isoelectric point. The isolated beta-1,4-mannanase showed activity against mannotetraose, mannopentaose and mannohexaose to produce mannobiose, mannotriose and mannotetraose. However, the enzyme exhibited no activity against mannobiose and mannotriose. Moreover, the crude enzyme preparation of the bacterium had no or minimal beta-mannosidase or alpha-galactosidase activity. Therefore, the enzyme preparation from P. polymyxa KT551 holds potential for the efficient production of mannooligosaccharides from natural resources of galactomannans.

• 出版日期2014-11