Development and UFLC-MS/MS Characterization of a Product Specific Standard for Phenolic Quantification of Maple-Derived Foods

作者：Liu Yongqiang; Ma Hang; Seeram Navindra P

来源：Journal of Agricultural and Food Chemistry, 2016, 64(17): 3311-3317.

DOI：10.1021/acs.jafc.6b01381

• maple
• phenolics
• lignans
• gallic acid equivalents (GAEs)
• Folin-Ciocalteu assay

The phenolic contents of plant foods are commonly quantified by the Folin-Ciocalteu assay based on gallic acid equivalents (GAEs). However, this may lead to inaccuracies because-gallic acid is not always representative of the structural heterogeneity of plant phenolics. Therefore, product-specific standards have been developed for the phenolic quantification of several foods. Currently, maple-derived foods (syrup, sugar, sap/water, and extracts) are quantified for phenolic contents based on GAEs. Because lignans are the predominant phenolics present in maple, herein, a maple phenolic lignan-enriched standard (MaPLES) was purified (by chromatography) and characterized (by UFLC-MS/MS with lignans previously isolated from maple syrup). Using MaPLES and secoisolariciresinol (a commercially available lignan), the phenolic contents of the maple-derived foods increased 3-fold compared to GAEs. Therefore, lignan-based standards are more appropriate for phenolic quantification of maple-derived foods versus GAEs. Also, MaPLES can be utilized for the authentication and detection of fake label claims on maple products.

• 出版日期2016-5-4