Association between DNA strand breakage and oxidative, inflammatory and endothelial biomarkers in type 2 diabetes

作者：Tatsch Etiane; Bochi Guilherme V; Piva Silvia J; De Carvalho Jose A M; Kober Helena; Torbitz Vanessa D; Duarte Thiago; Signor Cristiane; Coelho Adriane C; Duarte Marta M M F; Montagner Greice F F S; Da Cruz Ivana B M; Moresco Rafael N^*

来源：Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2012, 732(1-2): 16-20.

DOI：10.1016/j.mrfmmm.2012.01.004

• Type 2 diabetes
• DNA damage
• Comet assay

Evidence has been presented recently that type 2 diabetes patients have an increased level of DNA damage. This DNA damage could be associated with oxidative, inflammatory, and endothelial biomarkers and could represent a possible indication of injury in the endothelium and induction of inflammation in type 2 diabetes. To confirm this possible association, DNA strand breakage was evaluated by use of the comet assay and its association with oxidative, inflammatory, and endothelial biomarkers in type 2 diabetes patients. A case-control study (30 healthy controls and 32 subjects with type 2 diabetes) was performed to evaluate the association between DNA damage and NOx (nitrate/nitrite), interleukin-6 (IL-6), urinary albumin, fasting glucose, and glycated hemoglobin (HbA(1c)) levels. Type 2 diabetes patients presented higher DNA damage than control subjects, higher levels of IL-6 and urinary albumin, and lower NOx. Significant correlations between DNA damage and NOx (r = -0.303, p = 0.016), IL-6 (r = 0.845, p < 0.001), urinary albumin (r = 0.496, p < 0.001), fasting glucose (r = 0.449, p < 0.001), and HbA(1c), (r = 0.575, p < 0.001) were reported. Our findings showed an increase of DNA damage in type 2 diabetes especially in those patients with poor glycemic control and associations among NOx, IL-6 and urinary albumin levels with DNA damage.

• 出版日期2012-4-1